This guide provides a structured approach to the interpretation of pleural fluid results for specific diseases. Reference ranges vary between labs, so always consult your local medical school or hospital guidelines.
A pleural effusion is usually diagnosed on the basis of a chest X-ray. At least 300mL of fluid must be present before chest X-rays can detect a pleural effusion.
Once the accumulated fluid is more than 300 mL, clinical signs such as decreased chest-wall movement, dullness to percussion and diminished breath sounds on the affected side of the chest become evident. If there is a large effusion then tracheal deviation may occur away from the effusion. CT thorax is more accurate for diagnosis and is better at characterising the size and location of a pleural effusion.
Normal pleural fluid
Typical findings of normalpleuralfluid are as follows:
Protein: < 2% (1-2 g/dL)
White blood cells (WBC): < 1000/mm³
Glucose: similar to that of plasma
LDH: <50% plasma concentration
Amylase: 30-110 U/L
Triglycerides: <2 mmol/l
Cholesterol: 3.5–6.5 mmol/l
Transudate vs exudate
Transudative pleural effusions are defined as effusions that are caused by factors that alter hydrostaticpressure, pleuralpermeability, and oncoticpressure.
Conditions associated with transudative pleural effusions include:
Congestive heart failure
Exudative pleural effusions are caused by changes to the localfactors that influence the formation and absorption of pleuralfluid.
Conditions associated with exudative pleural effusions include:
Infection (e.g. empyema due to bacterial pneumonia)
Diagnostic criteria for pleural effusion
Protein <30 g/L (in patients with a normal serum protein level)
Protein >30 g/L (in patients with a normal serum protein level)
Light’s criteria are more accurate for the diagnosis of exudative effusions.
The fluid is considered an exudate if any of the following are present:
The ratio of pleural fluid to serum protein is greater than 0.5
The ratio of pleural fluid to serum LDH is greater than 0.6
The pleural fluid LDH value is greater than two-thirds of the upper limit of the normal serum value
If a patient is thought to have a transudative pleural effusion but the Light’s Criteria suggest an exudate, the serum–pleural fluid protein gradient should be examined.
Frankly purulentfluid indicates an empyema (an anaerobic empyema is likely if the fluid has a putrid odour).
A milkyfluid suggests chylothorax or pseudochylothorax. This is most often caused by lymphatic obstruction secondary to malignancy, chronic inflammation or thoracic duct injury by trauma or a surgical procedure.
Grossly bloodyfluid is usually associated with trauma. Less common causes include tuberculosis (TB), aorticdissection, ruptured aortic aneurysm and malignancy.
Straw-coloured fluid with the distinctive smell of ammonia is indicative of urinothorax.
The presence of foodparticles suggests oesophagealrupture.
Black pleural fluid is extremely rare, indicative of only a few diseases, including:
Aspergillus niger infection
Malignant melanoma (black colour caused by cells containing melanin pigment)
Haemorrhage and haemolysis associated with non-small cell lung cancer
Pleural fluid LDH
Levels greater than 1000 IU/L are suggestive of empyema, malignancy or rheumatoideffusion.
A low pleural fluid glucose level (<3.4 mmol/l) may be found in empyema, rheumatoid pleuritis and pleural effusions associated with TB, malignancy and oesophagealrupture.
A very low pleural glucose concentration (<1.6 mmol/l) is indicative of empyema and rheumatoiddisease.
A pleural fluid pH of less than 7.3 is associated with the same pathologies that cause low pleural fluid glucose levels.
In malignant effusions, a pleural fluid pH of less than 7.3 has been associated with more extensive pleural involvement and shorter life expectancies.
Pleural fluid amylase levels greater than 110U/L are indicative of pancreatitis, malignancy or a rupturedoesophagus.
White blood cells (WBC)
Results generally are not diagnostic, but most transudates have WBC counts less than 1000 cells/µL, whereas exudates generally have WBC counts greater than 50,000 cells/µL.
Pleural fluid lymphocytosis suggests TB, sarcoidosis or malignancy.
Neutrophildominanteffusions are associated with empyema or pulmonaryembolism.
Pleural fluid eosinophilia (PFE) is usually caused by the presence of air or blood in the pleural space.
Blood in the pleural space may be the result of pulmonary embolism or benign asbestosis. The presence of PFE does not exclude malignancy.
Cholesterol and triglycerides
Pleural fluid triglyceride levels greater than 1.24 mmol/l with a cholesterol level of less than 5.18 mmol/l is diagnostic of chylothorax.
A triglyceride level of less than 0.56 mmol/l with a cholesterol level of less than 5.18 mmol/l is associated with pseudochylothorax.
A 52-year-old male presents with a cough, shortness of breath and fever. CXR shows a right-sided pleural effusion. A thoracentesis is performed and the results of the pleural fluid analysis are as follows:
Pleural/serum total protein ratio: >0.5
WBC count: 67,000 cells/µL
Glucose: 1.5 mmol/l
LDH: 1430 IU/L
The most likely diagnosis is empyema. This gentleman has presented with, fever, shortness of breath and cough. The pleural fluid is purulent on inspection, the white cell count is significantly raised and glucose levels are low. The history and pleural fluid results are strongly suggestive of empyema and he should be treated empirically while culture results are awaited.
A 56 -year-old, previously well woman was admitted with a 4-week history of a cough, night sweats and 1-week history of progressive breathlessness. She had never smoked and has no history of heart disease or rheumatological disease. Pleural fluid results are as follows:
Colour: milky-white, odourless
Triglyceride: 0.5 mmol/l
Cholesterol: 12.4 mmol/l
The most likely diagnosis is pseudochylothorax. This woman has presented with a cough, night sweats and progressive breathlessness. A triglyceride level of less than 0.56 mmol/l and a cholesterol level of more than 5.18 mmol/l is indicative of pseudochylothorax. A pleural biopsy revealed chronic inflammation, and mycobacterium tuberculosis was isolated on a pleural fluid culture.
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